Figure 1.

Sensitivity of ovarian cancer cell lines to cytokines in vitro. Four human ovarian cancer cell lines were examined for their sensitivity to interferons (IFNs), tumor necrosis factor (TNF)‐α, and TNF‐related apoptosis‐inducing ligand (TRAIL) in vitro. A, SKOV3, B, ES2, C, A2780, and D, SW626 cells were cultured in 96‐well culture plates (3 × 10³ cells/well in 100 μL) with or without 30 ng/mL recombinant IFNs and TNFs. After 3 days, the number of living cells was measured by an MTS assay. The experiments were conducted three times to confirm the reproducibility of the assay, and representative results are presented. Data shown are the mean ± SD of triplicate assays. The difference from no cytokine condition was statistically significant (*P < 0.05; **P < 0.01 by Student's t test)