Figure 5.

Blockade of mutant isocitrate dehydrogenase 1 (IDH1) attenuated JQ1‐induced apoptosis in RBE cells. A, Viabilities of RBE cells after treatment for 48 h with the indicated doses of AGI‐5198 in combination with DMSO or JQ1 (1 μmol/L). Viability of each group was assessed relative to the DMSO‐treated control by using CCK‐8 assay. B, Effect of AGI‐5198 on caspase 3/7 activity in RBE cells. Cells were treated with vehicle or AGI‐5198 (20 μmol/L) in combination with DMSO or JQ1 (1 μmol/L) for 48 h. Data are presented as mean ± SEM (n = 4, *P < 0.05, ***P < 0.001, NS, not significant, Student's t test)