Figure 6.

Forced expression of mutant isocitrate dehydrogenase 1 (IDH1) enhanced the sensitivity of HuCCT1 cells to JQ1. A, Protein expression levels of total IDH1 and mutant IDH1 R132S were assessed by western blotting in HuCCT1 cells stably transfected with empty vector (EV), wild‐type IDH1 (Wild) and mutant IDH1 (R132S). B, Dose‐response curves show the effect of JQ1 on the growth of HuCCT1 cells stably transfected with wild‐type IDH1 (Wild) or mutant IDH1 (R132S). Cells were treated with JQ1 at the indicated concentrations for 72 h, and viability was assessed by using CCK‐8 assay. C, Caspase‐3/7 activity of HuCCT1 cells stably transfected with wild‐type IDH1 (Wild) or mutant IDH1 (R132S) were assessed after treatment with DMSO or JQ1 (1 μmol/L) for 48 h. DMSO control = 1. Data are presented as mean ± SEM (n = 4, *P < 0.05, **P < 0.01, NS, not significant, Student's t test)