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. 2018 Oct 6;109(11):3483–3493. doi: 10.1111/cas.13781

Figure 5.

Figure 5

The alternating magnetic field (AMF) inhibited Compound C‐induced autophagy and enhanced Compound C‐induced cytotoxicity in T98 cells. A, The representative western blot analysis showed that stimulation by Compound C for 24 h decreased the ratio of LC3BI/IIin T98 cells in a dose‐dependent manner. B, The representative western blot analysis showed that the AMF decreased Compound C‐induced autophagy in T98 cells in the presence of 5 μmol/L Compound C. C, The densitometric analysis showed that the AMF inhibited Compound C‐induced autophagy in T98 cells stimulated by 5 μmol/L Compound C (n = 4; ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001). D, The effect of chloroquine (CQ) (10 μmol/L) in cells stimulated by Compound C (5 μmol/L) for 24 h with/without the AMF. Proliferation was analyzed using the XTT assay (n = 4; ns, not significant;*P < 0.05, **P < 0.01, ***P < 0.001). E, Representative images of puncta in T98 cells in the presence of 5 μmol/L Compound C with/without the AMF for 30 min. F, The number of puncta in each cell (n = 50 cells; ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001)