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. 2018 Oct 15;109(11):3543–3553. doi: 10.1111/cas.13798

Figure 6.

Figure 6

Combinatorial transduction of HNF4A, HNF1A and FOXA3 induces hepatic differentiation of hepatocellular carcinoma (HCC) cells. A, The amounts of albumin secreted between day 7 and day 8 after transduction and CYP2C9 and CYP3A4 activities at day 9 after transduction were measured in cultures of HepG2 and HuH7 cells that expressed control EGFP or the combination of all 3 transcription factors (TF). Data represent means ± SD (n = 3). **P < .01. B, A GSEA of RNA‐seq data for HepG2 cells expressing all 3 TF (left panel) or EGFP (right panel) was performed using a set of genes specifically expressed in human hepatocytes or HepG2 cells. C, Enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway terms in the gene list of HepG2 cells expressing all 3 TF (left panel) or EGFP (right panel)