Figure 6.

Knockdown of androgen receptor (AR) decreases expression of AR coregulators KAT5 and downregulation of KAT5 promotes migration of prostate cancer cells. A, Gene expression of AR coregulators CREBBP, HSP90AA1, MDM2, NCOA3, NCOR1, NCOR2, SMARCA4, SP1, BRCA1, NR0B2, BAG1, KAT5, NCOA4, CAV1, GSN, and RACK1 in control and AR siRNA knockdown C4‐2B cells was determined by qRT‐PCR. Red and green color indicates increase and decrease of gene expression in AR siRNA knockdown C4‐2B cells as compared to control cells. B, Protein expression of KAT5 in nuclear extract of cell lysates in control and AR siRNA knockdown C4‐2B cells as determined by western blotting. Lamin A/C is used as loading control. C, Effect of siRNA knockdown of KAT5 or overexpression of KAT5 on migration of C4‐2B cells as compared to control cells. D, Effect of knockdown or overexpression of KAT5 on migration of C4‐2B cells with AR siRNA knockdown was determined by Transwell assay. Asterisks *, ** represent statistically significant difference P < 0.05, P < 0.01, respectively, between the two groups being compared