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. 2018 Sep 23;109(11):3611–3622. doi: 10.1111/cas.13788

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© 2018 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Resibufogenin (RB) inhibits cell viability and changes cell morphology. A, Chemical structure of RB. B, Cell viabilities in normal HPDE, Panc‐1, and Aspc cells treated with various concentrations of RB for the indicated times, determined by MTT. C, Cell morphology changes in HPDE, Panc‐1, and Aspc cells treatment with RB (5 μmol/L) for 48 hours were observed by microscope. Total numbers of live cells were counted and quantified as mean ± SD of three samples of each group. D, Colony formation showed the proliferation of Panc‐1 and Aspc cells treated with the indicated concentrations of RB. **P < .005, ***P < .0005; ^## P < .005, ^### P < .0005