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. 2018 Sep 23;109(11):3611–3622. doi: 10.1111/cas.13788

Figure 2.

Figure 2

Resibufogenin (RB) induces caspase‐dependent apoptosis in pancreatic cancer cells. A, Hoechst 33342 staining was used to analyze apoptotic cells in Panc‐1 and Aspc cells treated with RB (5 μmol/L) for 24 hours. B, Apoptosis of Panc‐1 and Aspc cells treated with RB for 24 hours, measured by flow cytometry. C, D, Levels of cleaved poly(ADP‐ribose) polymerase (PARP)1, caspase 9, and caspase 3 in Panc‐1 and Aspc cells treated with RB for 24 hours in the presence or absence of Z‐VAD‐FMK (20 μmol/L) were detected by immunoblotting. E, MTT was used to determine the cell viability in Panc‐1 and Aspc cells treated with RB (5 μmol/L) for 24 or 48 hours in the presence or absence of necrostatin‐1 (50 μmol/L). *P < .05, **P < 0.005; # P < .05, ## P < .005