Figure 5.

Resibufogenin (RB) suppresses transforming growth factor‐β‐activated kinase 1 (TAK1) and IκB kinase (IKK) activities in pancreatic cancer cells. A, B, Immunoblotting determined the levels of TAK1, TAB 1, and TAB 2 (A) and p‐IKKα/β, IKKα, p‐IκBα, and IκBα (B) in Panc‐1 and Aspc cells treated with 5 μmol/L RB for the indicated times. C, Flow cytometry analysis of apoptosis in Panc‐1 cells transfected with Flag‐TAK1 and TAB 1 following 5 μmol/L RB treatment for 24 hours. ^# P < .05, **P < .005