Figure 7.

Resibufogenin (RB) induces protein kinase C (PKC)‐mediated glycogen synthase kinase‐3 (GSK‐3) phosphorylation. A, B, Immunoblotting (IB) analyzing the levels of p‐Akt, p‐GSK‐3α, and p‐GSK‐3β in Panc‐1 cells pretreated with LY294002 (A) or Go6983 (B) for 1 hour following RB (5 μmol/L) treatment for 6 hours. C, IB determined the levels of p‐GSK‐3α and p‐GSK‐3β in Panc‐1 cells pretreated with indicated PKC inhibitors for 1 hour following RB (5 μmol/L) treatment for 6 hours. D, p‐PKCα/β levels in Panc‐1 cells treated with 5 μmol/L RB for the indicated times were determined by IB. E, IB analysis of p‐GSK‐3α, p‐GSK‐3β, p‐IKKα/β, and transforming growth factor‐β‐activated kinase 1 (TAK1) expression post‐siRNA knockdown of PKCβ in Panc‐1 cells