Figure 1. Effect of ex vivo silk peptide treatment of splenocytes on NK cell cytolytic activity against YAC-1 target cells. Splenocytes were incubated with the indicated concentrations of silk peptide for (A) 48 h or (B) 72 h and incubated with PKH-26-labeled YAC-1 target cells at a ratio of 10:1. The degree of target cell lysis was measured as described in the Materials and Methods. Data are presented as the mean±SE (n=3) of three independent experiments.

E:T, effector to target ratio.

^***p<0.001, ^****p<0.0001 indicate a significant difference compared to the control.