Figure 5. Level of cytokine expression following stimulation of splenocytes prepared from mice fed various amount of silk peptide with PMA and ionomycin. Splenocytes prepared from silk peptide-treated mice were stimulated in vitro with PMA and ionomycin. The expression levels of the Th1-type cytokines (A) IFN-γ and (B) IL-2, and the Th2-type cytokine (C) IL-4 were determined as described in the Materials and Methods. Data are presented as the mean±SE (n=3) of three independent experiments.

^*p<0.05 indicates a significant difference compared to the control (without silk peptide treatment).