Figure 4.

Anlotinib induces apoptosis in thyroid cancer cells. (A and D) BCPAP (A) and 8505C (D) cells were treated with DMSO and 10 µM anlotinib for 36 h. Cells were stained with Annexin V-FITC/PI and analyzed by flow cytometry. The low right (LR) quadrant of the histograms indicates the early apoptotic cells, and the upper right quadrant indicates the late apoptotic cells. (B and E) Quantification results of experiments described in A and D. Data are shown as mean ± s.d. (n ≥ 3). (C and F) BCPAP (C) and 8505C (F) cells were treated with anlotinib (0, 1, 5, 10 and 15 µM) for 36 h. Cell lysates were prepared and subjected to Western blot to detect the expression of TP53, cleaved caspase 3 (CL-caspase 3) and cleaved PARP (CL-PARP).