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. 2018 Oct 18;9:149–160. doi: 10.1016/j.isci.2018.10.015

Figure 1.

Figure 1

Doxycycline-Inducible Knockdown of MELK Impairs the Clonogenic Growth of TNBC Cells

(A) Fluorescent western blot analysis of MELK in BT549 cells that were stably transduced with tet-on-shMELK1 (Wang et al., 2014). Cells were either left untreated or treated with doxycycline (100 ng/mL) for the indicated time periods. The images were acquired via Odyssey CLx Infrared Imaging System (LI-COR Biosciences). Note that the first lane (M) was loaded with PageRuler Plus Prestained Protein Ladder (Thermo Fisher Scientific, cat# 26619), which have near-infrared fluorescence (molecular weight of the markers indicated).

(B) Fluorescent western blot analysis of MELK in BT549 and MCF7 cells that were stably transduced with tet-on-shMELK1. Cells were either left untreated or treated with doxycycline (100 ng/mL) for 2 days before lysate preparation and immunoblotting.

(C) Cells were seeded in 12-well plates at the indicated densities and were either left untreated or treated with doxycycline (100 ng/mL). Upon harvest, cells were fixed and stained with crystal violet. The staining was then extracted for quantification of cell growth (mean ± SD; n = 3).