Figure 2.

Identifying Guide Sequences for Efficient Gene Editing of MELK

(A) Schematic diagram of human MELK transcripts. The longest full-length one is isoform 1, shown at the bottom. The target locations of seven guide sequences (Lin et al., 2017), as well as the exon number of the MELK gene, are indicated.

(B) Fluorescent western blot analysis of MELK in MDA-MB-231 cells infected with control or MELK-targeting lentiCRISPR. Cells were harvested 7 days after infection. Total cell lysates were resolved on freshly cast 8% SDS-PAGE, transferred onto nitrocellulose membranes. Membranes were incubated with the indicated primary antibodies against MELK. The images were acquired using the Odyssey CLx Infrared Imaging System. Note that among the total seven guide sequences, sg_6 caused the most efficient reduction of MELK protein abundance.