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. 2018 Nov 1;20(12):1187–1197. doi: 10.1016/j.neo.2018.10.002

Figure 2.

Figure 2

krasV12 expression increases cell proliferation, Ras signaling, and EMT in the intestinal epithelium. Immunofluorescence staining (red) was carried out in intestine cross sections of wild-type and kras+ larvae at 8 dpf after 5 days of induction by 2 μM mifepristone. Only the intestine sections are shown. (A and B) Immunofluorescence staining of PCNA for cell proliferation (A) and quantification of percentage of positive cells (B). (C and D) Immunofluorescence staining of caspase 3 for apoptosis (C) and quantification of immunostaining density (D). (E and F) Immunofluorescence staining of p-Erk (E) and quantification of positive cells (F). (G and H) Immunofluorescence staining of E-cadherin (G) and quantification of immunostaining density (H). Immunostaining signals (D). Statistical significance: **P < .01, ****P < .0001.