Table 1.
List of antibiotics used in the experiments. The lower limit of quantification (LOQ) of the LC-MS/MS assay used is listed. The reference bacterium used for disc diffusion assays is also included.
| Antibiotic | Product Table | Supplier | LC-MS/MS LOQ | Disk Diffusion |
|---|---|---|---|---|
| Flucloxacillin | Floxapen® | Actavis, Regensdorf, Switzerland | 0.1 mg/L | S. aureus ATCC 25923 |
| Piperacillin+tazobactam | Tazobac® | Actavis, Regensdorf, Switzerland | Piperacillin 0.08 mg/LTazobactam 0.05 mg/L | E.coli ATCC 35218 |
| Cefuroxime | Zinacef® | GlaxoSmithKline, Münchenbuchsee, Switzerland | 0.1 mg/L | E. coli ATCC 25922 |
| Ceftriaxone | Rocephin® | Roche Pharma, Reinach, Switzerland | 0.5 mg/L | E. coli ATCC 25922 |
| Ceftazidime | Fortam® | GlaxoSmithKline, Münchenbuchsee, Switzerland | 0.1 mg/L | E. coli ATCC 25922 |
| Cefepime | Cefepime OrPha® | OrPha Pharma, Küsnacht, Switzerland | 0.05 mg/L | E. coli ATCC 25922 |
| Imipenem+cilastatin | Tienam® | MerckSharp&Dohme, Lucerne, Switzerland | Imipenem 0.05 mg/L | E. coli ATCC 25922 |
| Meropenem | Analytical quality | AstraZeneca, Grafenau, Switzerland | 0.05 mg/L | E. coli ATCC 25922 |
| Ciprofloxacin | Analytical quality | Bayer, Zürich, Switzerland | 0.1 mg/L | E. coli ATCC 25922 |
| Levofloxacin | Analytical quality | Sanofi-Aventis, Vernier, Switzerland | 0.1 mg/L | E. coli ATCC 25922 |
| Clindamycin | Analytical quality | Pfizer, Zürich, Switzerland | 0.1 mg/L | S. aureus ATCC 25923 |