Fig 4.

Influence of miR-543 on the malignant phenotypes of RCC in vitro. (A) CCK-8 assays were performed to determine cellular proliferation in A498 cells transfected with miR-543 inhibitors and 786-O cells transfected with miR-543 mimics. *P<0.05 vs. anti-NC group or NC group. (B) Cell cycle of A498 cells transfected with miR-543 inhibitors and 786-O cells transfected with miR-543 mimics were detected by flow cytometry. *P<0.05 vs. anti-NC group or NC group. (C, D) Transwell invasion assays were performed to examine migratory and invasive ability in A498 and 786-O cells after transfection. Cell number was counted in five random fields at 200 × magnification. *P<0.05 vs. anti-NC group or NC group. Data are presented as mean ± SD from three separated experiments.