Table 3.

Collaborative evaluation of the four multiplex cfDNA RMs (RMs L1-L4) by NGS

RMs	Gene	AA Change	Lab1	Lab2	Lab3	Lab4	Lab5	Lab6	Lab7	Lab8
L1	KRAS	G12S	2.92	3.07	2.26	2.11	2.83	2.35	2.22	1.30
	KRAS	G12C	2.13	2.82	2.22	3.53	ND	3.02	2.58	2.05
	KRAS	G12D	4.12	0.79	1.94	2.80	ND	2.31	2.20	1.90
	KRAS	G12A	3.30	3.08	2.84	3.20	2.94	3.38	3.40	2.28
	KRAS	G12V	2.68	2.02	2.23	3.27	ND	3.09	2.40	2.51
	KRAS	G13D	2.74	2.89	2.59	2.18	2.64	2.71	2.05	2.20
	NRAS	G12D	2.42	1.90	/	2.45	1.76	1.89	1.91	1.92
	NRAS	Q61R	2.93	2.40	/	2.86	ND	1.76	2.66	1.38
	NRAS	Q61K	4.01	5.50	/	5.13	5.13	5.28	4.60	5.02
	PIK3CA	H1047R	3.54	/	2.14	/	2.59	2.64	2.37	2.49
	BRAF	V600E	2.16	1.56	1.64	1.81	1.70	2.47	2.43	1.79
L2	KRAS	G12S	1.22	1.50	0.93	1.66	ND	1.69	1.03	0.74
	KRAS	G12C	1.83	1.90	1.53	2.20	1.56	1.53	2.04	1.57
	KRAS	G12D	0.91	0.63	1.62	1.91	ND	1.60	1.37	1.32
	KRAS	G12A	1.33	1.22	1.64	1.72	1.94	1.63	1.69	0.91
	KRAS	G12V	1.42	1.11	1.56	1.78	ND	1.56	1.34	0.98
	KRAS	G13D	0.57	1.59	1.44	1.49	0.83	1.53	1.26	0.54
	NRAS	G12D	1.44	0.77	/	0.71*	1.37	0.83	1.38	0.71
	NRAS	Q61R	1.48	1.59	/	1.22	ND	1.06	1.13	0.57*
	NRAS	Q61K	2.64	2.69	/	2.23	1.61	3.22	2.63	2.02
	PIK3CA	H1047R	0.87	/	1.47	/	1.53	1.58	1.27	1.15
	BRAF	V600E	1.15	0.93	0.93	0.76	0.66	1.34	1.06	0.89
L3	KRAS	G12S	0.42	0.79	0.63	1.31	ND	0.73	0.87	0.74
	KRAS	G12C	1.83	1.90	1.53	2.20	1.56	1.53	2.04	0.52*
	KRAS	G12D	1.01	0.33*	0.71	0.53*	ND	1.07	0.60	0.96
	KRAS	G12A	0.92	0.90	0.60	0.74	ND	0.85	0.57	0.64
	KRAS	G12V	0.31	0.58	0.57	0.61 *	1.08	1.16	0.80	0.96
	KRAS	G13D	0.89	0.85	0.61	0.84	0.75	0.38	0.81	0.50
	NRAS	G12D	0.90	0.59	/	0.77	0.38	0.58	0.60	0.52
	NRAS	Q61R	1.54	0.86	/	0.47*	ND	0.59	0.69	1.07
	NRAS	Q61K	1.51	1.60	/	0.89 *	1.61	1.53	1.19	1.08
	PIK3CA	H1047R	0.58	/	0.43	/	0.70	0.61	0.62	0.92
	BRAF	V600E	0.52	0.81	0.24	0.49 *	0.45	0.76	0.92	0.42
L4	KRAS	G12S	0.38	0.12*	0.13	ND	ND	0.08*	0.35	0.29*
	KRAS	G12C	0.10 *	0.20*	0.29	ND	0.31	0.28	0.29	0.35*
	KRAS	G12D	0.76	ND	0.29	0.19*	0.40	0.18 *	0.06	ND
	KRAS	G12A	0.10*	ND	0.09	0.19*	ND	0.12	0.00	ND
	KRAS	G12V	0.10	0.25*	0.13	ND	ND	0.16	0.19	0.07*
	KRAS	G13D	0.31*	ND	0.02*	0.18 *	ND	0.11*	0.09	0.45
	NRAS	G12D	0.18	0.06*	/	ND	0.13	0.03*	0.19*	0.23*
	NRAS	Q61R	0.09*	0.12*	/	ND	0.07*	0.01*	0.14	0.05*
	NRAS	Q61K	0.94	ND	/	ND	0.18	0.23	0.12	ND
	PIK3CA	H1047R	0.45	/	0.11	/	ND	0.14	0.15	0.13*
	BRAF	V600E	0.45	0.08*	0.10*	ND	ND	0.21	0.15*	0.15*

Note: The data was presented as average of two or three repetitions; /, not applicable in the panel; ND, not detected; *, different result from repetitions