Fig. 3.

APOBEC3B is stabilized in response to DSBs. (A–C) HeLa and SW480 cells were irradiated by γ-ray (A, C) or treated with hydroxyurea (B). The expression of APOBEC3B and HA-tagged APOBEC3B was analyzed by western blotting. Western blotting was performed using an anti-HA antibody to detect exogenous HA-tagged APOBEC3B in HeLa cells and an anti-APOBEC3B antibody to detect endogenous APOBEC3B in SW480 cells. Bars show means ± s.d. (n = 3 biologically independent experiments). (D) SW480 cells were treated with MG132 or PYR41 as indicated. Western blotting was performed using an anti-APOBEC3B antibody. (E) HeLa cells expressing HA-tagged APOBEC3B and the negative control were irradiated by γ-ray. Their survival efficiencies were assessed. The graph shows mean survival rate (%) ± s.d. (n = 3 biologically independent experiments).