Table 2.
Assessment of precision and accuracy of α-, β-, and γ-amanitin with and without internal standards (MetSSP and 15N10 α-amanitin) for QC Low (7.0 ng/mL) and QC Medium (75 ng/mL) in pooled urine for six calibration curves.
| α-amanitin | |||||||||
| Internal Standard | |||||||||
| None | MetSSP | 15N10-α-amanitin | |||||||
| QC Low | QC Medium | QC Low | QC Medium | QC Low | QC Medium | ||||
| Mean | 7.20 | 79.4 | 7.53 | 85.3 | 6.95 | 78.4 | |||
| CV | 13.0% | 8.75% | 17.5% | 8.01% | 3.96% | 1.51% | |||
| Accuracy | 103% | 107% | 108% | 114% | 99.3% | 105% | |||
| β-amanitin | |||||||||
| Internal Standard | |||||||||
| None | MetSSP | 15N10-α-amanitin | |||||||
| QC Low | QC Medium | QC Low | QC Medium | QC Low | QC Medium | ||||
| Mean | 6.99 | 77.3 | 7.07 | 84.5 | 6.76 | 76.4 | |||
| CV | 12.4% | 8.45% | 10.4% | 15.9% | 16.4% | 16.7% | |||
| Accuracy | 99.9% | 103% | 101% | 113% | 96.6% | 102% | |||
| ɣ-amanitin | |||||||||
| Internal Standard | |||||||||
| None | MetSSP | 15N10-α-amanitin | |||||||
| QC Low | QC Medium | QC Low | QC Medium | QC Low | QC Medium | ||||
| Mean | 7.05 | 79.2 | 7.47 | 85.6 | 6.97 | 77.6 | |||
| CV | 12.7% | 10.9% | 16.3% | 14.9% | 13.0% | 14.3% | |||
| Accuracy | 101% | 106% | 106% | 115% | 99.6% | 104% | |||