Fig. 1.

Of the ten triterpenoids isolated from Abies sp., two (700015 and 700234) had significant activity against Fasciola hepatica newly excysted juveniles (NEJs). F. hepatica NEJs (25 per well) were incubated with ten triterpenoids at a concentration of 10 μM (in 0.1% DMSO) and cultured for 72 h at 37 °C in an atmosphere containing 5% CO₂. Control wells (25 NEJs per well) included those containing 0.1% DMSO (negative) or 10 μM Triclabendazole (0.1% DMSO, positive). At 72 h, all NEJs were scored for phenotype (A) and motility (B) metrics as previously described (Edwards et al., 2015). *p = 0.05, **p = 0.01, ***p = 0.001.