Fig. 2.

Four triterpenoids, including 700015 and 700234, negatively affect Schistosoma mansoni schistosomula phenotype and motility metrics. Mechanically transformed schistosomula (120 per well) were cultivated in the presence of triterpenoids (10 μM in 0.625% DMSO) for 72 h at 37 °C in an atmosphere containing 5% CO₂. Control wells included schistosomula cultivated in DMSO (0.625%, negative), auranofin (in 0.625% DMSO, positive) and praziquantel (in 0.625% DMSO, positive). At 72 h, all schistosomula were scored for phenotype and motility as previously described (Paveley et al., 2012). Compounds were considered a hit (within the hit zone) when schistosomula phenotype fell below −0.15 and schistosomula motility fell below −0.35. The Z' values for this screen was 0.4 for motility and 0.5 for phenotype.