Fig. 4.

Dose response titration of the most active triterpenoid on Fasciola hepatica newly excysted juveniles (NEJs) and Schistosoma mansoni schistosomula. (A)F. hepatica NEJs (25 per well) were incubated with 700015 at concentrations of 10 μM, 5 μM, 2.5 μM, 1.25 μM and 0.625 μM (in 0.1% DMSO) and cultured for 72 h at 37 °C in an atmosphere containing 5% CO₂. A negative control well (25 NEJs per well) was included (0.1% DMSO) and is represented by −1.0 on the graphs. At 72 h, all NEJs were scored for phenotype and motility metrics. (B) Mechanically transformed schistosomula (120 per well) were cultivated in the presence of 700015 (10 μM, 5 μM, 2.5 μM, 1.25 μM and 0.625 μM in 0.625% DMSO) for 72 h at 37 °C in an atmosphere containing 5% CO₂. A control well included schistosomula cultivated in DMSO (0.625%, negative; represented by −1.0 on the graphs). At 72 h, all schistosomula were scored for phenotype and motility. The Z' values for this screen was 0.4 for motility and 0.5 for phenotype.