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. 2018 Oct 20;15(13):1530–1536. doi: 10.7150/ijms.28470

Figure 3.

Figure 3

Verification of VEGF expression and VEGF concentration in endometrial stromal cells treated for 24 hours with 10% peritoneal fluid followed by 24-hour mistletoe treatment. VEGF expression verified by western blot analysis in eutopic endometrial stromal cells (A), and in ectopic endometrial stromal cells (B). VEGF concentration was measured by ELISA in eutopic endometrial stromal cells (C) and in ectopic endometrial stromal cells (D) (CON: media only for 24h, served as a standard, M: 200 ng/mL of mistletoe for 24h, CP: 10% control peritoneal fluid for 24h, CP+M: 10% of control peritoneal fluid for 24h, then treated with 200 ng/mL mistletoe for 24h, G1: 10% of peritoneal fluid from endometriosis stage I & II patients for 24h, G1+M: 10% of peritoneal fluid from endometriosis stage I & II patients for 24h, then treated with 200 ng/mL mistletoe for 24h, G2: 10% of peritoneal fluid from endometriosis stage III & IV patients for 24h, G2+M: 10% of peritoneal fluid from endometriosis stage III & IV patients for 24h, then treated with 200 ng/mL mistletoe for 24h * P < 0.05).