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. 2018 Oct 20;15(13):1537–1546. doi: 10.7150/ijms.24296

Figure 4.

Figure 4

Tid1S overexpression inhibits ISO-induced NFATc3 nuclear translocation. H9c2 cells were transfected with different amounts of Tid1S plasmid (1 and 3μg) for 24 hours, and then treated with ISO (5*10-5 M) in serum-free medium for 24 hours. (a) Total cell lysates analyze the phospho- (cytosol) and dephospho-NFATc3 (nuclear) by immunoblotting. (b) The nuclear translocation of NFATc3 were measured using confocal microscopy. NFATc3 is shown in green fluorescence and nuclei were counter stained with DAPI (blue fluorescence).