Fig. 4.

The effects of arsenite on the [Ca²⁺]_cand [Ca²⁺]_mare reversible: prolonged exposure to the metalloid is required to promote a sustained increase of the concentration of the cation in mitochondria, necessary for mitochondrial dysfunction and apoptosis. RP-, D- and RD-cells were treated for 6 h with arsenite (Treatment A). Cells received Treatment A and were then incubated for a further 10 h in fresh culture (Treatment B). Cells were treated for 16 h with arsenite (Treatment C). After treatments, the cells were analyzed for Fluo- 4- (A), Rhod-2- (B), MitoSOX (C) or NAO- (D) fluorescence. Results represent the means ± SD calculated from 3 to 6 separate experiments.