Fig. 6.
Sustained increase in the [Ca2+]mleads to apoptosis. Cells were treated as indicated in the legend to Fig. 4, in which a definition of Treatment A-C is also provided. Treatment D refers to cells exposed to the metalloid for 48 h. RP-cells received Treatment D also in the presence of 2-APB, Ry or Ru360. After treatments, the cells were analyzed for caspase-3 activity (A), or for apoptosis, by measuring chromatin fragmentation/condensation with the Hoechst assay (B). Results represent the means ± SD calculated from 5 separate experiments. *P < 0.01, as compared to untreated cells. (*)P < 0.01, as compared to cells treated with arsenite (one-way ANOVA followed by Dunnet's test).