Table 1.

Effect of various treatments on the DHR-fluorescence caused by H₂O₂.

Treatment	DHR-fluorescence (arbitrary units)
Untreated RP-cells	14.5 + 1.4
H2O2	28.9 + 2.5*
+ Rotenone	29.6 + 2.2*
+ AA	27.4 + 2.4*
+2-APB	30.1 + 3.3*
+ Ry	27.7 + 2.7*
+ Ru360	29.0 + 2.6*

RP-cells were treated for 15 min with 0 or 50 µM H₂O_2, in the absence or presence of rotenone (0.5 µM), 2-APB (50 µM), Ry (20 µM) or Ru360 (10 µM). Drugs were added to the cultures 5 min prior to H₂O₂. AA (10 µM) was given to the cells 15 min prior to H₂O_2. After treatments, the cells were analyzed for DHR-fluorescence. Results represent the means ± SD calculated from at least 3 separate experiments.

^*P < 0.001; as compared to untreated cells (one-way ANOVA followed by Dunnett's test).