Fig. 2.
Treatment with exogenous irisin attenuates organ injury after hepatic ischemia-reperfusion (I/R). Irisin treatment in mice was conducted by intravenous administration (250 μg/kg, a single dose) at the beginning of reperfusion. The liver, lung and blood samples were harvested at 24 h after reperfusion. A, Hematoxylin and eosin staining (H&E) of representative liver sections (magnification 100× and 200×); B, Percentage of necrotic areas; C, Liver histological scores; D-G, The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactic dehydrogenase (LDH) and lactate in each group, respectively;H, Hematoxylin and eosin staining (H&E) of representative lung sections (magnification 200×); I, Lung injury scores. n = 6, mean ± SEM, *P < 0.05 versus sham group, #P < 0.05 versus vehicle group.