. 2018 Aug 31;21:763–766. doi: 10.1016/j.dib.2018.08.167

Table 2.

Nucleotide sequences of primers and conditions used to amplify species specific, virulence markers and antibiotic resistance genes in E.coli isolates by PCR.

Virulence factor	Target gene	Primer name	Sequence (5′ to 3′)	Length (bp)	Annealing temperature (°C)	Amplicon size (pb)	References
traT	traT	traT-F	GGTGTGGTGCGATGAGCACAG	21	60	290	[11]
traT	traT	traT-R	CACGGTTCAGCCATCCCTGAG	21	60	290	[11]
fimH	fimH	fimH-F	CATTCGCCTGTAAAACCGCC	20	60	207	[12]
fimH	fimH	fimH-R	ATAACACGCCGCCATAAGCC	20	60	207	[12]
blaCTX	blaCTX	blaCTX-F	TTTGCGATGTGCAGTACCAGTAA	23	55	544	[12]
blaCTX	blaCTX	blaCTX-R	CGATATCGTTGGTGGTGCCATA	23	55	544	[12]
tetA	tetA	tetA-F	TTGGCATTCTGCATTCACTC	20	60	494	[9]
tetA	tetA	tetA-R	GTATAGCTTGCCGGAAGTCG	20	60	494	[9]
blaTEM	blaTEM	TEM-F	ATAAAATTCTTGAAGACGAAA	19	50	1150	[13]
blaTEM	blaTEM	TEM-R	GACAGTTACCAATGCTTAATCA	19	50	1150	[13]
blaSHV	blaSHV	SHV-F	CACTCAAGGATGTATTGTG	19	50	885	[10]
blaSHV	blaSHV	SHV-R	TTAGCGTTGCCAGTGCTCG	19	50	885	[10]