Figure 4.

Assessment of Cardiac Pathology in D2/mdx Mice Administered AAV-μ-Utro Compared to Vehicle Control Mice

Representative images of (A) muscle architecture (assessed by H&E), (B) calcification (red; assessed by alizarin red; AR), (C) fibrosis (assessed by Masson’s trichrome; MT; indicated in blue) of cardiac cross-sections from treated mice compared to vehicle control mice. Quantification of (F) AR and (G) MT, expressed as percentage area. Representative images and quantification of (H) β-dystroglycan (β-DG) and (J) phosphorylated and total GSK-3β (GSK) assessed by western blot. β-DG was normalized to loading control (total protein; TPS). Scale bar, 100 μm. Data represented as mean ± SEM, *p < 0.05 versus vehicle control mice, n = 8/group.