Fig. 1.

Effect of tamoxifen treatment on L. amazonensis sphingolipid biosynthesis. Metabolic labeling of 1 × 10⁸L. amazonensis promastigotes with 6 μCi of [³H]-sphingosine (lanes 2 and 3) or 6 μCi of [³H]-inositol (lanes 5 and 6). Parasites were treated with 10 μM tamoxifen for 4 h and purified total lipids were analyzed by HPTLC using running system 1. ST(S): [³H]-sphingosine standard without incubation with cells, ST(I): [³H]-inositol standard without incubation with cells, Sph*: lipid extract of [³H]-sphingosine labeled parasites, Ino*: lipid extract of [³H]-inositol labeled parasites, Ct: lipid extract of untreated parasites, Tam: lipid extract of parasites treated with tamoxifen, C16-cer: C16-ceramide standard, PI 18:1: phosphatidylinositol 18:1 standard, O: origin, F: front.