Fig. 2.

Lipid B characterization by alkaline hydrolysis. Lipid B fractions purified from HPTLC plates after separation of total lipids from L. amazonensis promastigotes metabolic labeled with 2 μCi [³H]-sphingosine. Initial lipid extracts were obtained from parasites treated with 10 μM tamoxifen or left untreated. Eluted lipids were incubated in 100 mM NaCl (lanes 1 and 2) or in 100 mM NaOH (lanes 3 and 4) in 95% methanol aqueous solution for 1 h. Extracts from 3 × 10⁸ cells were applied to each lane and analyzed by HPTLC using running system 2. Ct: lipid extract of untreated parasites, Tam: lipid extract of parasites treated with tamoxifen, NaCl: extract incubated with 100 mM NaCl, NaOH: extract incubated with 100 mM NaOH, O: origin, F: front.