Fig. 9.

Sphingolipid biosynthesis in L. amazonensis promastigotes treated with tamoxifen.L. amazonensis promastigotes (4 × 10⁸) were treated with tamoxifen and metabolic labeled with 6 μCi [³H]-inositol (1–6) or 2 μCi [³H]-sphingosine (7–10). Parasites were supplemented with 0.2 (2, 5, 9) or 2 mM (3, 6, 10) myo-inositol. Total lipid extracts from 1 × 10⁸ cells were spotted on each lane and analyzed by HPTLC using running system 1. Tam: 10 μM tamoxifen for 4 h; Sph*: labeling with [³H]-sphingosine; Ino*: labeling with [³H]-inositol; Ino: unlabeled inositol; O: origin; F: front.