Table 2.
L. amazonensis inositol transporter Km and Vmax after treatment with tamoxifen for 10 min at 25 °C.
| Tamoxifen (μM) | Kma [μM (CI95%)] | Vmaxb [pmol min−1 3 × 107 cells−1(CI95%)] |
|---|---|---|
| 0 | 182.9 (155.0–210.9) | 76.5 (72.9–80.2) |
| 10 | 193.9 (137.2–250.6) | 66.8 (60.8–72.7) |
| 30 | 203.2 (146.4–260.0) | 63.7 (58.0–69.4) |
| 50 | 197.7 (141.1–254.4) | 50.9 (46.6–55.2) |
a
Michaelis-Menten constant expressed in micromolar and 95% confidence interval.
b
Maximum velocity of inositol uptake expressed in picomoles per minute per 3 × 107 cells and 95% confidence interval.