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. 2018 Aug 1;27(22):3911–3918. doi: 10.1093/hmg/ddy286

Figure 1.

Figure 1

CRISPR/Cas9-mediated generation of Dmc1 M200V mice. (A) Diagram of human DMC1 protein labeled with known functional domains and binding sites. The M200V amino acid change is encoded by SNP rs2227914. (B) CRISPR-Cas9 genome editing strategy to introduce the M200V amino acid change. Underlined is the sgRNA sequence, in bold is the PAM site, highlighted in yellow are nucleotide changes for Val and italicized is the restriction enzyme site for Hpy188I (TCN/GA). (C) Sanger sequencing chromatograms from WT (left) and Dmc1M200V/M200V mouse (right).