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V566 has less kinase activity than D566. (A)Perk-/- MEFs were transfected with PERK(p566Asp) or PERK(p566Val) and treated with Tg at the indicated concentration for 1 h. Protein lysates were immunoblotted for PERK, phosphorylated PERK (P-PERK), P-eIF2α and GAPDH (loading control). Representative immunoblots are shown (n = 5).
