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. 2018 Aug 21;27(22):3951–3963. doi: 10.1093/hmg/ddy297

Figure 4.

Figure 4

Generation of iPSC-derived neuronal cultures from PSP patients. (A) Images of representative iPSCs expressing pluripotency markers, Oct3/4, Sox2 and Nanog. Scale bar is 100 μm. (B) EB cultures derived from iPSC were stained with endodermal marker, α-fetoprotein (AFP), mesodermal marker, smooth muscle actin and neuroectodermal markers, Map2b or β-III-tubulin. Scale bar is 50 μm. (C) Representative G-banded normal karyotype from an iPSC line. (D) Representative images of FACS-purified iPSC-derived neural stem cells expressing NSC marker Sox2. Scale bar is 50 μm. (E) Representative images of 4 week differentiated neural cultures immunofluorescently stained with neuronal markers Map2b, β-III-tubulin or astrocytic marker glial fibrillary acid protein. Scale bar is 50 or 25 μm as shown.