Figure EV2. Notch1 receptor expression was not changed by Ttyh1 overexpression.

• A, B
  Two days after E14.5 primary neural progenitor cells were transduced with retroviral vectors expressing Ttyh1, Notch1 mRNA (A) and protein (B) levels were assessed by qPCR. Error bars represent SD. Student's t‐test was used to determine statistical significance. NS, not significant. n = 3 for (A).
• C
  E14.5 neural progenitor cells were transduced with Ttyh1 retroviruses. At 2 days posttransduction, a representative FACS histogram showing cell surface levels of Notch1 was obtained using an anti‐Notch1 antibody (Invitrogen, MA5‐11961). The lightly shaded curve represents the secondary antibody‐only control.

Source data are available online for this figure.