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. 2018 Sep 3;19(11):e45472. doi: 10.15252/embr.201745472

Figure 4. Cl− channel property of Ttyh1 is not associated with Notch pathway‐enhancing activity.

Figure 4

  • A, B
    Effects of the ion selectivity‐perturbing R371Q mutation in (A) Ttyh1 and (B) other Ttyh family members on Notch‐dependent transcription. E14.5 neural progenitor cells were transduced with retroviral vectors expressing each indicated gene, and 2 days posttransduction, cells were harvested for qPCR analyses of Notch target mRNAs (n = 3).
  • C, D
    (C) Immunolabeling of E15.5 brain sections electroporated in utero with indicated plasmids at E13.5 using anti‐GFP and Alexa Fluor 488‐conjugated secondary antibodies. GFP immunofluorescence (green) was merged with DAPI‐counterstained images (blue). (D) Quantification of GFP+ cell localization in (C) (n = 3). VZ, ventricular zone; SVZ, subventricular zone; IZ, intermediate zone; CP, cortical plate. Scale bar: 100 μm.
  • E, F
    (E) Schematic representation of a series of Ttyh1 mutants with deletions in the C‐terminal cytoplasmic tail region (Ttyh1 gene structure was not drawn in proportion to the actual domain size), and (F) their effects on cell distribution assessed by intraventricular in utero electroporation at E13.5 followed by immunolabeling at E15.5 using anti‐GFP antibody. Scale bar: 100 μm.
Data information: Error bars represent SD. Student's t‐test was used to determine statistical significance. *P < 0.05, **P < 0.01, ***P < 0.001.