Figure 2.

Generation of beating cardiomyocytes from pluripotent cells derived from human amniotic fluid. (A): Representative confocal microscopy images of cardiomyocyte subtypes exhibiting comparable mixed immunostaining patterns of sarcromeric proteins, including ventricle (MLC2v, FITC secondary, green) and other cardiac‐specific markers, including MYH7, MF20, MLC2a, and TTNT2 (magnification, ×40). (B): Representative pseudocolor activation map of intracellular calcium transient propagation in amniotic fluid cardiomyocyte monolayers showing typical radial spreading patterns after spontaneous activation. Isochrone lines (right) indicate differential activation times. (C): Representative calcium transient recordings after β‐adrenergic agonist (50 nM ISO) exposure of amniotic fluid cardiomyocytes demonstrating appropriate chronotropic responses compared to baseline. Modified from 35 with permission. Abbreviation: ISO, isoproterenol.