The proportion of spvA ON cells in populations of the indicated strains grown to stationary phase in LB (A) or ISM (B). Cells of the corresponding populations were analyzed with fluorescence microscopy and binned as ON when their mVenus fluorescence exceeded 10 A.U., with this cut-off being based on visual inspection of the raw microscopy images. The data show the mean proportion of spvA ON cells in the total population with the corresponding SEM from three biological replicates. The number of cells quantified for every biological replicate of each strain in both growth media was in the range of 800–2200 cells. One-way ANOVA, followed by Tukey’s honest significant difference test, was performed on the two separate data sets to test for statistically significant differences between the strains (* P < 0.05, ** P < 0.01, *** P < 0.001).