Figure 3.

Cellular uptake of (A) PTXD-Se NPs and uPA-PTXD NPs with uPA modifying ratios of (B) 10, (C) 20, and (D) 40 wt% in MDA-MB-231 cells after 30 min incubation. (E-I) Possible endocytosis pathway of uPA-PTXD NPs in this study. Cells were blocked by the inhibitors (E) 20 μM uPA peptide, (F) 1 μg/mL filipin, (G) 0.3 μg/mL PhAsO, (H) 1 μg/mL Colchicine, and (I) cells were incubated at 4 °C. Scale bars represent 100 μm. (J) Quantitative results of cellular uptake and endocytosis pathway from flow cytometry analysis.