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. 2018 Oct 31;11:7679–7690. doi: 10.2147/OTT.S173378

Figure 4.

Figure 4

(A) Scratch assay of RCC cells treated with the indicated concentration of CD for 8 and 20 hours. (B) Transwell migration assay of RCC cells treated with 0.1% DMSO, 10 or 20 µM, and 40 µM CD for 24 hours. Scale bar =50 µm. 50× magnification. (C) Matrigel invasion assay of RCC cells treated with 0.1% DMSO or 40 µM CD for 24 hours. Scale bar =50 µm. 100× magnification.

Notes: The bar graphs show the quantification of cell numbers that passed through the Transwell membrane from three independent experiments. *P<0.05, **P<0.01, ***P<0.001 compared with the control (0 µM group).

Abbreviations: CD, 16-hydroxycleroda-3,13-dien-15,16-olide; DMSO, dimethyl sulfoxide; RCC, renal cell carcinoma.