Table 1.
Antibody | Dilution | Brand |
---|---|---|
Anti-vimentin (2707), anti- pSrc (Tyr418; ab133460), anti-pNF-κB (Ser536; ab76302), anti-glycerol-3-phosphate dehydrogenase (GAPDH; ab9484), p21 (ab109520) | WB: 1:1,000 IF: 1:50 | Epitomics (an Abcam company, Burlingame, CA, USA) |
Anti-paxillin (610051), anti- pFAK (Tyr397; 611722), anti-FAK (610087), p53 (554293) | WB: 1:1,000 IF: 1:50 | BD Biosciences (San Jose, CA, USA) |
Anti-cyclin-dependent kinase (CDK) 2, anti-CDK4, anti-cyclin B1, anti-cyclin D1, anti-vascular endothelial growth factor (VEGF, sc-152) | 1:500 | Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) |
Anti-cyclin E1 (GTX103045) anti-MMP-2 (GTX104577), anti-MMP-9 (GTX100458) | 1:1,000 1:500 1:500 | GeneTex, Inc. (Irvine, CA, USA) |
Anti-vinculin (700062) | 1:1,000 | Thermo Fisher Scientific (Waltham, MA, USA) |
Abbreviations: IF, immunofluorescence; MMP, matrix metalloproteinase; pFAK, phosphorylated focal adhesion kinase; WB, Western blotting.