Fig. 2.

Impact of R256 mutation on bicarbonate enhancement of SLAC1-mediated currents. (A) Representative whole-cell Cl⁻ current recordings in oocytes coexpressing SLAC1yc or SLAC1-R256Ayc with OST1yn. Currents were recorded in response to 3-s voltage pulses ranging from +40 mV to −160 mV in −20 mV steps, with a holding potential at 0 mV followed by −120 mV after voltage pulses. (B) Mean current–voltage curves of oocytes coexpressing the indicated proteins with or without NaHCO₃ injection. (C) Average currents of the indicated SLAC1 isoforms with and without injection of 11.5 mM bicarbonate (14, 22, 29) at −160 mV. The bath solution contained 10 mM MES/Tris (pH = 7.4), 1 mM MgCl₂, 1 mM CaCl₂, 2 mM HCl, 24 mM NaCl, and 70 mM sodium gluconate. Three independent batches of oocytes showed similar results (n = 9 to 14 oocytes from each oocyte batch per condition). Error bars denote mean ± SEM. Means with letters (a, b, and c) are grouped based on one-way ANOVA and Tukey’s test, P < 0.05.