Fig. 7.

Effect of point mutation SLAC1-R256A on HCO₃⁻ activation of S-type anion channel currents in guard cells of slac1-transformed lines. (A) Representative current recordings in whole-cell patch clamp configuration with guard cell protoplasts from 4- to 6-wk-old WT and transformed slac1 lines. (B) Current-voltage relationships of WT and the indicated transformed slac1 lines in the absence (control) or presence of added HCO₃⁻ in the cytosol. WT control guard cells and SLAC1-R256A#7 control cells without addition of HCO₃⁻ showed only small background currents that overlap with the illustrated controls and are not included as data points in B for clarity. Error bars show SEM. Note that Figs. 7 and 8 share the same control current–voltage curves, as they were completed within the same datasets.