Fig. 2.

Human aromatase expression and estrogen production lead to LAM tissue fibrosis, myocyte atrophy, and the development of scrotal hernia in Arom^hum mice. (A) The incidence of scrotal hernia. Scrotal hernia formation was monitored by weekly visual inspection from 3 to 26 wk of age. No scrotal hernia was seen in the WT group. n = 32 mice. (B) The area of scrotal hernia was measured from 3- to 26-wk-old mice. Two-tailed Student’s t test, **P < 0.01 for Arom^hum vs. WT, n = 32 mice. (C) H&E and (D) Masson’s trichrome staining of UAM and LAM from 26-wk-old WT and Arom^hum mice. n = 10 mice for C and D. Yellow arrows in C and D indicate one of many atrophied myocytes. (Scale bars, 100 μm; Magnification: Inset in C, 40×.) Quantification of the percentage of connective tissue or fibrotic area (E) or myofiber area (F) in WT and Arom^hum mice. Ten representative high-power fields were analyzed in each tissue. Two-way ANOVA with Tukey’s multiple comparison test, *P < 0.05, **P < 0.01, n = 8 mice per group.