Fig. 6.

Androgen action is lower in LAM of Arom^hum mice. (A) Serum T levels in WT and Arom^hum mice were measured by radioimmunoassay. Mouse sera were collected from 26-wk-old mice. Two-tailed Student’s t test, *P < 0.05, n = 15. (B) Total AR mRNA levels (AR-FL and AR45) in UAM and LAM tissues of WT and Arom^hum mice. Two-way ANOVA with Tukey’s multiple comparison test, n = 10. (C) Nuclear AR-FL assessed by IHC in UAM and LAM of WT and Arom^hum mice. n = 8. (Scale bar, 50 μm; Magnification: C, 40×.) (D) AR-FL and AR45 protein levels in UAM and LAM tissues of WT and Arom^hum mice. GAPDH protein levels served as the loading control. Human prostate cancer cell line LNCaP served as AR-FL and AR45 positive controls. Quantification of AR-FL and AR45 by densitometry in UAM and LAM of WT (E) and Arom^hum (F) mice. Two-way ANOVA with Sidak’s multiple comparison test, **P < 0.01, n = 4 mice in each group. mRNA levels of androgen-responsive genes Amd2 (G), Cyp2e1(H), and Inmt1 (I) in UAM and LAM tissues of WT and Arom^hum mice. GAPDH mRNA levels served as the control. Two-way ANOVA with Tukey’s multiple comparison test, *P < 0.05, n = 10 mice per group.